abstract：:MicroRNAs (miRNAs) are increasingly implicated in the regulation of metastasis. Despite their potential as targets for anti-metastatic therapy, miRNAs have only been silenced in normal tissues of rodents and nonhuman primates. Therefore, the development of effective approaches for sequence-specific inhibition of miRNA...

journal_title：Nature biotechnology

authors： Ma L,Reinhardt F,Pan E,Soutschek J,Bhat B,Marcusson EG,Teruya-Feldstein J,Bell GW,Weinberg RA

更新日期：2010-04-01 00:00:00

abstract：:Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-termini of their protease cleavage products would accelerate identification of the substrates of proteases with broad or unknown specificity. Our approach, named terminal amine isotopic labeling of substrates (TAILS), addresses th...

journal_title：Nature biotechnology

authors： Kleifeld O,Doucet A,auf dem Keller U,Prudova A,Schilling O,Kainthan RK,Starr AE,Foster LJ,Kizhakkedathu JN,Overall CM

更新日期：2010-03-01 00:00:00

abstract：:Replicate mass spectrometry (MS) measurements and the use of multiple analytical methods can greatly expand the comprehensiveness of shotgun proteomic profiling of biological samples. However, the inherent biases and variations in such data create computational and statistical challenges for quantitative comparative a...

journal_title：Nature biotechnology

authors： Griffin NM,Yu J,Long F,Oh P,Shore S,Li Y,Koziol JA,Schnitzer JE

更新日期：2010-01-01 00:00:00

abstract：:Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...

journal_title：Nature biotechnology

authors： Asokan A,Conway JC,Phillips JL,Li C,Hegge J,Sinnott R,Yadav S,DiPrimio N,Nam HJ,Agbandje-McKenna M,McPhee S,Wolff J,Samulski RJ

更新日期：2010-01-01 00:00:00

abstract：:Increasing the in vivo residence times of protein therapeutics could decrease their dosing frequencies. We show that genetic fusion of an unstructured recombinant polypeptide of 864 amino acids, called XTEN, to a peptide or protein provides an apparently generic approach to extend plasma half-life. Allometric scaling ...

journal_title：Nature biotechnology

authors： Schellenberger V,Wang CW,Geething NC,Spink BJ,Campbell A,To W,Scholle MD,Yin Y,Yao Y,Bogin O,Cleland JL,Silverman J,Stemmer WP

更新日期：2009-12-01 00:00:00

abstract：:Efficient delivery of small interfering (si)RNA to specific cell populations in vivo remains a formidable challenge to its successful therapeutic application. We show that siRNA synthetically linked to a CpG oligonucleotide agonist of toll-like receptor (TLR)9 targets and silences genes in TLR9(+) myeloid cells and B ...

journal_title：Nature biotechnology

authors： Kortylewski M,Swiderski P,Herrmann A,Wang L,Kowolik C,Kujawski M,Lee H,Scuto A,Liu Y,Yang C,Deng J,Soifer HS,Raubitschek A,Forman S,Rossi JJ,Pardoll DM,Jove R,Yu H

更新日期：2009-10-01 00:00:00

abstract：:Analysis of abnormally methylated genes is increasingly important in basic research and in the development of cancer biomarkers. We have developed methyl-BEAMing technology to enable absolute quantification of the number of methylated molecules in a sample. Individual DNA fragments are amplified and analyzed either by...

journal_title：Nature biotechnology

authors： Li M,Chen WD,Papadopoulos N,Goodman SN,Bjerregaard NC,Laurberg S,Levin B,Juhl H,Arber N,Moinova H,Durkee K,Schmidt K,He Y,Diehl F,Velculescu VE,Zhou S,Diaz LA Jr,Kinzler KW,Markowitz SD,Vogelstein B

更新日期：2009-09-01 00:00:00

abstract：:As the cost of human genome sequencing plunges and large-scale genome-phenotype studies become possible, society should do more to reward those individuals who choose to disclose their data, despite the risks. ...

journal_title：Nature biotechnology

authors：

更新日期：2009-09-01 00:00:00

abstract：:RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we re...

journal_title：Nature biotechnology

authors： Eguchi A,Meade BR,Chang YC,Fredrickson CT,Willert K,Puri N,Dowdy SF

更新日期：2009-06-01 00:00:00

abstract：:Influenza A virus leads to yearly epidemics and sporadic pandemics. Present prophylactic strategies focus on egg-grown, live, attenuated influenza vaccines (LAIVs), in which attenuation is generated by conferring temperature sensitivity onto the virus. Here we describe an alternative approach to attenuating influenza ...

journal_title：Nature biotechnology

authors： Perez JT,Pham AM,Lorini MH,Chua MA,Steel J,tenOever BR

更新日期：2009-06-01 00:00:00

abstract：:We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces ac...

journal_title：Nature biotechnology

authors： Hosaka T,Ohnishi-Kameyama M,Muramatsu H,Murakami K,Tsurumi Y,Kodani S,Yoshida M,Fujie A,Ochi K

更新日期：2009-05-01 00:00:00

abstract：:Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets. We report a method to specifically capture an arbitrary subset of genomic targets for single-molecule bisulfite sequencing for digital quantification of DNA methylation at single-nucleotide...

journal_title：Nature biotechnology

authors： Deng J,Shoemaker R,Xie B,Gore A,LeProust EM,Antosiewicz-Bourget J,Egli D,Maherali N,Park IH,Yu J,Daley GQ,Eggan K,Hochedlinger K,Thomson J,Wang W,Gao Y,Zhang K

更新日期：2009-04-01 00:00:00

abstract：:Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor ...

journal_title：Nature biotechnology

authors： Markoulaki S,Hanna J,Beard C,Carey BW,Cheng AW,Lengner CJ,Dausman JA,Fu D,Gao Q,Wu S,Cassady JP,Jaenisch R

更新日期：2009-02-01 00:00:00

abstract：:Efficient analysis of very large amounts of raw data for peptide identification and protein quantification is a principal challenge in mass spectrometry (MS)-based proteomics. Here we describe MaxQuant, an integrated suite of algorithms specifically developed for high-resolution, quantitative MS data. Using correlatio...

journal_title：Nature biotechnology

authors： Cox J,Mann M

更新日期：2008-12-01 00:00:00

abstract：:Most microbes in the biosphere remain unculturable. Whole genome shotgun (WGS) sequencing of environmental DNA (metagenomics) can be used to study the genetic and metabolic properties of natural microbial communities. However, in communities of high complexity, metagenomics fails to link specific microbes to specific ...

journal_title：Nature biotechnology

authors： Kalyuzhnaya MG,Lapidus A,Ivanova N,Copeland AC,McHardy AC,Szeto E,Salamov A,Grigoriev IV,Suciu D,Levine SR,Markowitz VM,Rigoutsos I,Tringe SG,Bruce DC,Richardson PM,Lidstrom ME,Chistoserdova L

更新日期：2008-09-01 00:00:00

abstract：:An analysis of US Federal Circuit decisions shows strategies for designing around patents. ...

journal_title：Nature biotechnology

authors： Wang SJ

更新日期：2008-05-01 00:00:00

abstract：:Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...

journal_title：Nature biotechnology

authors： Tafer H,Ameres SL,Obernosterer G,Gebeshuber CA,Schroeder R,Martinez J,Hofacker IL

更新日期：2008-05-01 00:00:00

abstract：:Development of a cell therapy for diabetes would be greatly aided by a renewable supply of human beta-cells. Here we show that pancreatic endoderm derived from human embryonic stem (hES) cells efficiently generates glucose-responsive endocrine cells after implantation into mice. Upon glucose stimulation of the implant...

journal_title：Nature biotechnology

authors： Kroon E,Martinson LA,Kadoya K,Bang AG,Kelly OG,Eliazer S,Young H,Richardson M,Smart NG,Cunningham J,Agulnick AD,D'Amour KA,Carpenter MK,Baetge EE

更新日期：2008-04-01 00:00:00

abstract：:St. Louis wants to become a hub of agricultural biotechnology. All it needs is more start-ups and funds. ...

journal_title：Nature biotechnology

authors： Marris E

更新日期：2008-04-01 00:00:00

abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...

journal_title：Nature biotechnology

authors： Giordano-Coltart J,Calkins CW

更新日期：2008-02-01 00:00:00

abstract：:Tissue function depends on hierarchical structures extending from single cells ( approximately 10 microm) to functional subunits (100 microm-1 mm) that coordinate organ functions. Conventional cell culture disperses tissues into single cells while neglecting higher-order processes. The application of semiconductor-dri...

journal_title：Nature biotechnology

authors： Khetani SR,Bhatia SN

更新日期：2008-01-01 00:00:00

abstract：:Live cell imaging is a powerful method to study protein dynamics at the cell surface, but conventional imaging probes are bulky, or interfere with protein function, or dissociate from proteins after internalization. Here, we report technology for covalent, specific tagging of cellular proteins with chemical probes. Th...

journal_title：Nature biotechnology

authors： Fernández-Suárez M,Baruah H,Martínez-Hernández L,Xie KT,Baskin JM,Bertozzi CR,Ting AY

更新日期：2007-12-01 00:00:00

abstract：:RNA interference (RNAi) is a conserved sequence-specific, gene-silencing mechanism that is induced by double-stranded RNA. RNAi holds great promise as a novel nucleic acid-based therapeutic against a wide variety of diseases, including cancer, infectious diseases and genetic disorders. Antiviral RNAi strategies have r...

journal_title：Nature biotechnology

authors： Haasnoot J,Westerhout EM,Berkhout B

更新日期：2007-12-01 00:00:00

abstract：:Academic researchers have regularly ignored patents on key technologies as a strategy to maneuver around patent thickets and freedom-to-operate issues, but they may be more at risk than they realize. ...

journal_title：Nature biotechnology

authors： Yancey A,Stewart CN Jr

更新日期：2007-11-01 00:00:00

abstract：:In vitro reprogramming of somatic cells into a pluripotent embryonic stem cell-like state has been achieved through retroviral transduction of murine fibroblasts with Oct4, Sox2, c-myc and Klf4. In these experiments, the rare 'induced pluripotent stem' (iPS) cells were isolated by stringent selection for activation of...

journal_title：Nature biotechnology

authors： Meissner A,Wernig M,Jaenisch R

更新日期：2007-10-01 00:00:00

abstract：:Thermostable enzymes combine catalytic specificity with the toughness required to withstand industrial reaction conditions. Stabilized enzymes also provide robust starting points for evolutionary improvement of other protein properties. We recently created a library of at least 2,300 new active chimeras of the biotech...

journal_title：Nature biotechnology

authors： Li Y,Drummond DA,Sawayama AM,Snow CD,Bloom JD,Arnold FH

更新日期：2007-09-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is an essential regulator of normal and abnormal blood vessel growth. A monoclonal antibody (mAb) that targets VEGF suppresses tumor growth in murine cancer models and human patients. We investigated cellular and molecular events that mediate refractoriness of tumors to anti-a...

journal_title：Nature biotechnology

authors： Shojaei F,Wu X,Malik AK,Zhong C,Baldwin ME,Schanz S,Fuh G,Gerber HP,Ferrara N

更新日期：2007-08-01 00:00:00

abstract：:A wealth of molecular interaction data is available in the literature, ranging from large-scale datasets to a single interaction confirmed by several different techniques. These data are all too often reported either as free text or in tables of variable format, and are often missing key pieces of information essentia...

journal_title：Nature biotechnology

authors： Orchard S,Salwinski L,Kerrien S,Montecchi-Palazzi L,Oesterheld M,Stümpflen V,Ceol A,Chatr-aryamontri A,Armstrong J,Woollard P,Salama JJ,Moore S,Wojcik J,Bader GD,Vidal M,Cusick ME,Gerstein M,Gavin AC,Superti-Furga G,

更新日期：2007-08-01 00:00:00

abstract：:Paralleling the diversity of genetic and protein activities, pathologic human tissues also exhibit diverse radiographic features. Here we show that dynamic imaging traits in non-invasive computed tomography (CT) systematically correlate with the global gene expression programs of primary human liver cancer. Combinatio...

journal_title：Nature biotechnology

authors： Segal E,Sirlin CB,Ooi C,Adler AS,Gollub J,Chen X,Chan BK,Matcuk GR,Barry CT,Chang HY,Kuo MD

更新日期：2007-06-01 00:00:00

abstract：:Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanog...

journal_title：Nature biotechnology

authors： Brunner E,Ahrens CH,Mohanty S,Baetschmann H,Loevenich S,Potthast F,Deutsch EW,Panse C,de Lichtenberg U,Rinner O,Lee H,Pedrioli PG,Malmstrom J,Koehler K,Schrimpf S,Krijgsveld J,Kregenow F,Heck AJ,Hafen E,Schlapbach R

更新日期：2007-05-01 00:00:00

abstract：:Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical pract...

journal_title：Nature biotechnology

authors： Liu QP,Sulzenbacher G,Yuan H,Bennett EP,Pietz G,Saunders K,Spence J,Nudelman E,Levery SB,White T,Neveu JM,Lane WS,Bourne Y,Olsson ML,Henrissat B,Clausen H

更新日期：2007-04-01 00:00:00

abstract：:Biological systems are controlled by protein complexes that associate into dynamic protein interaction networks. We describe a strategy that analyzes protein complexes through the integration of label-free, quantitative mass spectrometry and computational analysis. By evaluating peptide intensity profiles throughout t...

journal_title：Nature biotechnology

authors： Rinner O,Mueller LN,Hubálek M,Müller M,Gstaiger M,Aebersold R

更新日期：2007-03-01 00:00:00

abstract：:Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy (CLEM), a simple imagi...

journal_title：Nature biotechnology

authors： Hoebe RA,Van Oven CH,Gadella TW Jr,Dhonukshe PB,Van Noorden CJ,Manders EM

更新日期：2007-02-01 00:00:00

abstract：:The nonprofit Pew Initiative on Food and Biotechnology is closing, but the need for an independent and neutral body to facilitate dialog on US biotech policy has never been greater. ...

journal_title：Nature biotechnology

authors：

更新日期：2007-02-01 00:00:00

abstract：:Debates on patenting DNA must evolve to reflect the global decline in filings and regional disparities in patenting activity. ...

journal_title：Nature biotechnology

authors： Hopkins MM,Mahdi S,Patel P,Thomas SM

更新日期：2007-02-01 00:00:00

abstract：:Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified fo...

journal_title：Nature biotechnology

authors： Mallick P,Schirle M,Chen SS,Flory MR,Lee H,Martin D,Ranish J,Raught B,Schmitt R,Werner T,Kuster B,Aebersold R

更新日期：2007-01-01 00:00:00

abstract：:Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...

journal_title：Nature biotechnology

authors： Lewis K,Ausubel FM

更新日期：2006-12-01 00:00:00

abstract：:For the past five decades, the need for new antibiotics has been met largely by semisynthetic tailoring of natural product scaffolds discovered in the middle of the 20(th) century. More recently, however, advances in technology have sparked a resurgence in the discovery of natural product antibiotics from bacterial so...

journal_title：Nature biotechnology

authors： Clardy J,Fischbach MA,Walsh CT

更新日期：2006-12-01 00:00:00

abstract：:Of paramount importance for the development of cell therapies to treat diabetes is the production of sufficient numbers of pancreatic endocrine cells that function similarly to primary islets. We have developed a differentiation process that converts human embryonic stem (hES) cells to endocrine cells capable of synth...

journal_title：Nature biotechnology

authors： D'Amour KA,Bang AG,Eliazer S,Kelly OG,Agulnick AD,Smart NG,Moorman MA,Kroon E,Carpenter MK,Baetge EE

更新日期：2006-11-01 00:00:00

abstract：:Antifreeze proteins (AFPs) are found in cold-adapted organisms and have the unusual ability to bind to and inhibit the growth of ice crystals. However, the underlying molecular basis of their ice-binding activity is unclear because of the difficulty of studying the AFP-ice interaction directly and the lack of a common...

journal_title：Nature biotechnology

authors： Doxey AC,Yaish MW,Griffith M,McConkey BJ

更新日期：2006-07-01 00:00:00